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Gary Schroth, PhD presents an introduction to index hopping, points out how to minimize index hopping levels by pursuing library prep ideal methods, and discusses how to eliminate index-hopped reads employing special twin indexing mixtures.
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Go ahead and take guesswork out of the upcoming workflow. Get tips and methods so that you can sequence with assurance.
For the i5 index, evaluate shade harmony utilizing the ahead orientation for MiSeq runs and MiniSeq runs with immediate reagents, as well as reverse-complement orientation for all other devices.
Take the guesswork out of the upcoming workflow. Get recommendations and sources so you're able to sequence with assurance.
A fast, integrated workflow for preparing libraries to be used in a variety of sequencing purposes.
Create full-genome sequencing libraries and proficiently interrogate samples with confined offered DNA.
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Therefore these application Focus on the assumption that the i5 sequence is often inside the ahead orientation and will routinely create the reverse complement of your sequences offered for the suitable instrument type.
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The sequences shown are what exactly is entered into the final sample sheet. Package definitions hold the i5 within the ahead orientation. The i5 sequences might be properly reverse complemented and displayed during the IEM interface based upon instrument/reagent variety.